CHEM 370: Instrumental Analysis I

CHEM 370 - Instrumental Analysis (Lecture) is an introduction to common techniques of instrumental analysis. It is designed to prepare students for careers as chemical and/or forensic analysts and will further introduce them to the field of analytical chemistry as it relates to chemical instrumentation. Students will study the theoretical, electrical, and mechanical underpinnings of the most common methods of instrumental analysis. Topics covered include spectroscopy, mass spectrometry, separation science, and statistical treatment of data. Grades of C- or better in both CHEM-232 (Quantitative Analysis) and CHEM-242 (Organic Chemistry II) are prerequisite to this course.

Workshops & Readings

You will receive paper copies of the workshop assignments to complete in class.

Unit 1: Foundations

Reading List

All readings come from Instrumental Analysis by David Harvey.

• Chapter 1: Introduction

• Appendix A: Statistical Treatment of Data

• Chapter 5: Signals and Noise

Workshops

1. Review (not done in class)

2. Statistics

3. Standardization



Unit 2: Spectroscopy

Reading List

All readings come from Instrumental Analysis by David Harvey.

• Chapter 6: Introduction

• Chapter 7: Components of Optical Instruments

• Chapter 13: UV-vis Absorption

• Chapter 14: Applications of UV-vis

• Chapter 15: Molecular Luminescence

• Chapter 16: Introduction to IR

• Chapter 17: Applications of IR

• Chapter 8: Introduction to Atomic Spectroscopy

• Chapter 9: Atomic Absorption

• Chapter 10: Atomic Emission

Workshops

1. Molecular Origins of Absorption (POGIL)

2. Optical Instrument Design

3. Quantitative Applications and Atomic Spectroscopy

4. Beer's Law

5. Types of Spectrometers and Components

Unit 3: Mass Spectrometry

Reading List

All readings come from An Introduction to Mass Spectrometry by Van Bramer.

• Chapter 1: Introduction

• Sections 3.1, 3.2, 3.4, 3.6: Ionization Techniques

• Sections 4.1, 4.2, 4.3, 4.4: Mass Analyzers

• Chapter 5: Mass Spectrometer Systems

• Chapter 6: Interpretation

Workshops & Practice Problems

1. Workshop 1 - Mean Free Path and ICP-MS

2. Workshop 1 - Mean Free Path and ICP-MS - KEY

3. Workshop 2 - Interpretation and ICP-MS

4. Workshop 2 - Interpretation and ICP-MS - KEY

5. MS Practice A

6. MS Practice B

7. MS Practice C

8. MS Practice D

See also the sections/chapters containing practice problems!

Unit 4: Chromatography

Reading List

All readings come from Instrumental Analysis by David Harvey.

• Chapter 26: Introduction to Chromatography

• Chapter 27: Gas Chromatography

• Chapter 28: HPLC